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Researchers are adding probiotics to chocolate to make it even healthier

Chocolate is already a beloved treat, but what if it could also improve your gut health?

Alexandra Gerea
March 14, 2025 @ 11:23 am

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broken pieces of chocolate
AI generated image of chocolate.

Chocolate already carries an aura of indulgence. It melts on your tongue, delivers a hit of dopamine, and—if it’s dark enough—offers antioxidants that fight cellular damage. But what if it could also boost your gut health? A new study, published in ACS Food Science & Technology, suggests just that.

Researchers infused chocolate with probiotics—the beneficial bacteria often associated with yogurt and kimchi—along with prebiotics, the fibers that help these microbes survive and thrive. Their goal was to create a “synbiotic” treat that’s not only delicious but also packed with digestive benefits.

Ultimately, they created a chocolate that delivers live bacteria to your gut, maintains their probiotic punch for months, and—most importantly—still tastes good.

Probiotics + Chocolate = Love

Probiotics are important for maintaining gut health, which in turn influences nearly every system in the body. The trillions of bacteria in your digestive tract—collectively known as the gut microbiome—help break down food, produce essential vitamins, and regulate immune function. They even play a role in your mental health. A well-balanced microbiome can reduce inflammation, improve digestion, and even support mental health by influencing neurotransmitters like serotonin.

Most probiotic foods take the form of tangy dairy products, fermented vegetables, or beverages with a bit too much fizz. Some people love these foods, but many don’t. Chocolate, on the other hand, is beloved across cultures and demographics. If probiotics could hitch a ride in a food people actually crave, they might be more widely consumed.

This isn’t the first attempt at probiotic chocolate. Previous studies have explored ways to incorporate these microbes, but the process can be tricky. Probiotics are fragile, easily killed by heat, moisture, and stomach acid. That’s where prebiotics—substances like fiber and certain sugars—come in. They shield probiotics from environmental threats and help them survive the journey through the digestive tract.

How Does Probiotic Chocolate Hold Up?

The researchers, led by Smriti Gaur and Shubhi Singh from the Jaypee Institute of Information Technology in India, crafted five versions of chocolate. One was a traditional recipe—just cocoa butter, cocoa powder, and milk powder—while the other four contained different combinations of probiotics, prebiotics, and flavors. Then they ran tests to see how these chocolates stacked up.

Gaur and Singh opted for two strains of bacteria, Lactobacillus acidophilus La-14 and Lactobacillus rhamnosus GG, both well-documented for their gut health benefits. To keep the process simple, they chose natural prebiotics—corn and honey—along with cinnamon and orange for flavor.

Some findings were as expected: fat levels, which affect the melt-in-your-mouth quality of chocolate, stayed consistent across all samples. But other changes stood out. For instance, the orange-flavored chocolates had higher moisture levels, lower pH, and more protein than the others. Meanwhile, the probiotic-enhanced chocolates boasted stronger antioxidant properties than the control sample.

But the most impressive result was how long the probiotics lasted. These chocolates stayed microbiologically viable for 125 days—longer than many previous attempts at embedding probiotics in chocolate. Even after exposure to simulated stomach conditions, the probiotic count remained above a key threshold for gut health benefits.

But Does It Taste Good?

Of course, none of this matters if the chocolate doesn’t taste good. The researchers didn’t conduct a larger-scale taste test, but they did try it out themselves. They were pleased with the results.

“Personally, we enjoyed the orange-flavored chocolates the most, where the vibrant citrus notes complemented the rich cocoa, and it had a slightly softer texture that made each bite feel more luxurious,” says Gaur.

If they can bring the price down to a competitive level, these chocolates could become competitive as a healthier alternative, but only if the product tastes good.

Probiotics in chocolate aren’t entirely new, but previous attempts haven’t always nailed the taste. Some probiotic strains can impart bitterness or chalky textures. If this method avoids those pitfalls while delivering genuine health benefits, it could stand a better chance of making it to store shelves.

But will people embrace a chocolate that markets itself as gut-friendly rather than just indulgent? That’s a question that researchers will focus on in the future.

“In the future, we are excited to explore additional health benefits of these chocolates while thoroughly investigating their sensory and nutritional profiles, with the goal of creating an even more wholesome and enjoyable treat.”

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